Evaluation of Gene Expression Level of miRNA-29c, miRNA-125, miRNA-141, miRNA-145 and miRNA-205 as Predisposing Factors for Transitional Cell Carcinoma-Bladder Cancer in Iraqi Patients

MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression post-transcriptionally. Transitional cell carcinoma(TCC) of the bladder Cancer (C) account 95 percent malignancies, with males having a greater prevalence than females. The current study sought to determine whether there is link between miRNA-29c, miRNA-125, miRNA-141, miRNA-145 and miRNA205 levels TCC/BC risk in Iraqi cancer patients. In prospective cross-sectional investigation, 149 samples were collected (95 urine 54 tissue biopsies). From November 2018 August 2019, 37/95 randomly taken from healthy persons. Total RNA was extracted samples, then converted cDNA via reverse transcription. Quantitate Real-Time-PCR done using specific primers for quantification level studied miRNAs. results showed 32/49 (65%) patients had non-muscles invasive (stage T1), while 17/49 (35%) muscles T2-T4). Fold change miRNA-125 highly significant differences (T1) invasion (T2-T4) both biopsies Chi-square test at p ≤ 0.01. Whereas miR-29c, miR-141, miR-145 miR-205 no > 0.05 samples. Thus, miR-125 can be associated development stages TCC-BC as an increase during final stage cancer.Hence, this could considered good predictor advanced cancer.